History of DNA Sequencing

The sequence of sequencers: The history of sequencing DNA

Determining the order of nucleic acid residues in biological samples is an integral component of a wide variety of research applications. Over the last fifty years large numbers of researchers have applied themselves to the production of techniques and technologies to facilitate this feat, sequencing DNA and RNA molecules. This time-scale has witnessed tremendous changes, moving from sequencing...

DNA sequencing: clinical applications of new DNA sequencing technologies.

We are in a time of great change in genetics that may dramatically impact human biology and medicine. The completion of the human genome project,1,2 the development of low-cost, high-throughput parallel sequencing technology, and large-scale studies of genetic variation3 have provided a rich set of techniques and data for the study of genetic disease risk, treatment response, population diversi...

DNA Sequencing

Double stranded DNA sequencing as a choice for DNA sequencing.

Double stranded DNA sequencing (1) is favored because of its simplicity, and convenice. However it is only recently that the quality of this method has become comparable with single stranded DNA sequencing. We believe that two factors have limited the popularity of double stranded DNA sequencing: 1, the quality of the template DNA; 2, the inherent propertv.,of the DNA polymerase. The modified T...

A DNA ‘Barcode Blitz’: Rapid Digitization and Sequencing of a Natural History Collection

DNA barcoding protocols require the linkage of each sequence record to a voucher specimen that has, whenever possible, been authoritatively identified. Natural history collections would seem an ideal resource for barcode library construction, but they have never seen large-scale analysis because of concerns linked to DNA degradation. The present study examines the strength of this barrier, carr...